serpine1 virus Search Results


sh serpine1  (Genechem)
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Genechem sh serpine1
SERPINE1 promotes the PDAC process in vitro . (A) Western blot analysis of SERPINE1 protein levels in PANC-1 and CFPAC-1 cells with SERPINE1 knockdown. (B, C) Viability of PANC-1 and CFPAC-1 cells transfected with <t>sh-SERPINE1.</t> (D) Calcein-AM/PI staining of cell death rate in PANC-1 and CFPAC-1 cells with SERPINE1 knockdown. (E) Western blot analysis of cleaved caspase-3 in PANC-1 and CFPAC-1 cells. (F) Protein levels of mTOR, p-mTOR, S6K, and p-S6K in PANC-1 and CFPAC-1 cells after SERPINE1 knockdown. (G) Colony-formation assay showing proliferation in PANC-1 and CFPAC-1 cells with SERPINE1 knockdown. (H) Wound healing assay showing migration in PANC-1 and CFPAC-1 cells with SERPINE1 knockdown ***p < 0.001; **0.001 < p < 0.01; *0.01 < p < 0.05; ns p > 0.05.
Sh Serpine1, supplied by Genechem, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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SERPINE1 promotes the PDAC process in vitro . (A) Western blot analysis of SERPINE1 protein levels in PANC-1 and CFPAC-1 cells with SERPINE1 knockdown. (B, C) Viability of PANC-1 and CFPAC-1 cells transfected with sh-SERPINE1. (D) Calcein-AM/PI staining of cell death rate in PANC-1 and CFPAC-1 cells with SERPINE1 knockdown. (E) Western blot analysis of cleaved caspase-3 in PANC-1 and CFPAC-1 cells. (F) Protein levels of mTOR, p-mTOR, S6K, and p-S6K in PANC-1 and CFPAC-1 cells after SERPINE1 knockdown. (G) Colony-formation assay showing proliferation in PANC-1 and CFPAC-1 cells with SERPINE1 knockdown. (H) Wound healing assay showing migration in PANC-1 and CFPAC-1 cells with SERPINE1 knockdown ***p < 0.001; **0.001 < p < 0.01; *0.01 < p < 0.05; ns p > 0.05.

Journal: Frontiers in Immunology

Article Title: Integrated multi-omics and single-cell analysis identify SERPINE1 as a key mediator of the inflammatory tumor microenvironment in PDAC

doi: 10.3389/fimmu.2025.1716878

Figure Lengend Snippet: SERPINE1 promotes the PDAC process in vitro . (A) Western blot analysis of SERPINE1 protein levels in PANC-1 and CFPAC-1 cells with SERPINE1 knockdown. (B, C) Viability of PANC-1 and CFPAC-1 cells transfected with sh-SERPINE1. (D) Calcein-AM/PI staining of cell death rate in PANC-1 and CFPAC-1 cells with SERPINE1 knockdown. (E) Western blot analysis of cleaved caspase-3 in PANC-1 and CFPAC-1 cells. (F) Protein levels of mTOR, p-mTOR, S6K, and p-S6K in PANC-1 and CFPAC-1 cells after SERPINE1 knockdown. (G) Colony-formation assay showing proliferation in PANC-1 and CFPAC-1 cells with SERPINE1 knockdown. (H) Wound healing assay showing migration in PANC-1 and CFPAC-1 cells with SERPINE1 knockdown ***p < 0.001; **0.001 < p < 0.01; *0.01 < p < 0.05; ns p > 0.05.

Article Snippet: Lentiviral vectors for sh-Ctrl and sh-SERPINE1 were designed and synthesized by Genechem Co., Ltd. (Shanghai, China).

Techniques: In Vitro, Western Blot, Knockdown, Transfection, Staining, Colony Assay, Wound Healing Assay, Migration